OMICS 18(3): 184-95).ĭCA Divide-and-Conquer Multiple Sequence Alignment ( Universitat Bielefeld, Germany) - is a program for producing fast, high quality simultaneous multiple sequence alignments of amino acid, RNA, or DNA sequences. It combines two processes: a tool that converts the huge NGS mapping or coverage files into light specific coverage files containing information on genetic elements and a visualization interface allowing a real-time analysis of data with optional integration of statistical results. pii: giy169).ĬOV2HTML: a visualization and analysis tool of bacterial next generation sequencing (NGS) data for postgenomics life scientists - allows performing both coverage visualization and analysis of NGS alignments performed on prokaryotic organisms (bacteria and phages). The results contain alignment statistics, consensus sequence, polymorphisms data, and visualization of the alignment. ( Reference: Shabardina V et al. The range of the target species/sequences for the alignment is not limited, and the descriptive usage page of NanoPipe helps a user to succeed with NanoPipe analysis. NanoPipe - was developed in consideration of the specifics of the MinION sequencing technologies, providing accordingly adjusted alignment parameters. Currently, they are able to integrate datasets below 20 Mb of contigs per bin. Metagenome project: Annotation and comparative analyses of assembled metagenomic sequences. For pre-annotated sequences, they only integrate annotations from NCBI RefSeq complete genome section. Genome project: Annotation and comparative analyses of finished or draft genome sequences. MicroScope web site (hosted at Genoscope), provides an environment for expert annotation and comparative genomics. 9: 868-877), and here.ĬAP EST Assembler (Istituto FIRC di Oncologia Molecolare, Italy) - Maximum sequence length for each sequence is 30 kb - Maximum number of sequences 10 kb Research in Computational Molecular Biology: pp 158-170).ĬAP3 ( PBIL, France ), ( Reference: Huang,X. 50(4): 1355-1361).ĬGE SPAdes 3.9 - assembles Illumina and Ion Torrent data ( Reference: S. 34: W459-462).ĬGE Assembler 1.2 - assembles Illumina, 454, SOLid and Ion Torrent data ( Reference: Larsen MV, et al. Sequence assembly - you don't need your own contig assembly program when you can use:ĮGassember - aligns and merges sequence fragments resulting from shotgun sequencing or gene transcripts (EST) fragments in order to reconstruct the original segment or gene ( Reference: A. Specialized annotation - Genomic IslandsĭNA Sequence Quality - Phred - provides base calling, chromatogram display and high quality sequence region evaluation and presentation for up to five sequences simultaneously. Specialized annotation - virulence determinantsġ1. Specialized annotation - antibiotic resistanceġ0. Two-component and other regulatory proteinsĨ. Specialized annotation - general (inteins, plasmids, typing, vaccine candidates)Ħ. Here we have unique tools for genomic analysis which do not fit easily in that section.ĥ. N.B.Many of the tools that one needs for the analysis of genomes can be found in the DNA Sequence Analysis section.
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